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United States Department of Agriculture

Agricultural Research Service

Title: RT-PCR DETECTION OF AVIAN PNEUMOVIRUS STORED ON FTA INDICATING CLASSIC CARD

Authors
item Thayer, Stephan - UNIV OF GEORGIA-ATHENS,GA
item Kapczynski, Darrell
item Senne, Dennis - USDA/ARS/NVSL-AMES,IA
item Moscoso, Hugo - UNIV OF GEORGIA-ATHENS,GA
item Hofacre, Charles - UNIV OF GEORGIA-ATHENS,GA

Submitted to: American Association of Avian Pathologists
Publication Type: Abstract Only
Publication Acceptance Date: June 12, 2005
Publication Date: July 20, 2005
Citation: Thayer, S.G., Kapczynski, D.R., Senne, D., Moscoso, H., Hofacre, C. 2005. Rt-pcr detection of avian pneumovirus stored on fta indicating classic card. In: Proceedings of the American Association of Avian Pathologists, July 16-20, 2005, Minneapolis, Minnesota. p.77.

Technical Abstract: Much work has been done on demonstrating inactivation and performing time/temperature studies to permit shipment under USDA permit of avian pathogens on Whatman FTA Indicating Filter paper cards for subsequent DNA/RNA analysis. The work presented here continues with the addition of Avian Pneumovirus (APV) to the list of transportable pathogens. This work demonstrates that APV can be applied to Whatman FTA Indicating Filter paper cards and the RNA extracted and detected by RT-PCR the same day(0), 4, 7, 10, and 14 days post-FTA inoculation on cards stored at room temperature and 37C. Reisolation studies are under way to confirm inactivation of APV on the FTA.

Last Modified: 10/31/2014
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