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United States Department of Agriculture

Agricultural Research Service

Title: Calculation of Rates for Enzyme and Microbial Kinetics Via a Spline Technique

Author
item Klasson, K Thomas

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: September 18, 2005
Publication Date: October 19, 2005
Citation: Klasson, K.T. 2005. Calculation of rates for enzyme and microbial kinetics via a spline technique (abstract). International Symposium on Biocatalysis and Biotechnology: Functional Food and Industrial Products. Paper No. K-2.

Technical Abstract: In biocatalysis research, determination of enzyme kinetics, microbial growth rates, substrate utilization rates, and product accumulation rates sometime require derivatives to be calculated with a method that can be duplicated and yields consistent results. In this paper, several methods that have been suggested in the literature are discussed and the result is presented graphically. A spreadsheet methodology was developed to handle the complicated equations. The results show that a cubic spline method proved more versatile in its use, even though the spreadsheet programming was challenging. The methods discussed were tested with published literature data for cell growth, and a preferred technique was developed based on smoothing the data and using this smooth data for rate calculations.

Last Modified: 7/31/2014
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