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United States Department of Agriculture

Agricultural Research Service

Title: DNA Fingerprint Diversity among Brucella Isolates Recovered from Wildlife

item Bricker, Betsy
item Ewalt, Darla - USDA, APHIS,NVSL

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: September 11, 2005
Publication Date: October 15, 2005
Citation: Bricker, B.J., Ewalt, D.R. 2005. DNA Fingerprint Diversity Among Brucella Isolates Recovered From Wildlife [abstract]. 58th Brucellosis Research Conference. p. 59.

Technical Abstract: DNA fingerprinting of Brucella isolates using HOOF-Print analysis is based on elevated mutation rates in genomic regions containing short tandem repeats. Last year we reported on the analyses of numerous B. abortus isolates, recovered from US outbreaks in cattle occurring over the last 15 years. The results of the survey revealed considerable genotypic diversity among the HOOF-Print profiles. In this study, we extend the analysis to Brucella isolates from wildlife species. The results show that there is considerable genetic diversity among wildlife isolates as well. We intend to use these results, and future ones, in creating an international DNA fingerprint database for Brucella isolates as a resource for the research community. Key words: HOOF-Prints, genotype, wildlife, epidemiology

Last Modified: 4/22/2015
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