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United States Department of Agriculture

Agricultural Research Service

Title: Developing Transcriptional Profiles and Gene Expression Analysis of Grape Plant Response to Xylella Fastidiosa

Authors
item Lin, Hong
item Doddapaneni, Harsha - UNIV OF CALIF-DAVIS
item Walker, Andrew - UNIV OF CALIF-DAVIS

Submitted to: CDFA Pierce's Disease Control Program Research Symposium
Publication Type: Proceedings
Publication Acceptance Date: October 1, 2005
Publication Date: December 5, 2005
Citation: Lin, H., Doddapaneni, H., Walker, A. 2005. Developing Transcriptional Profiles and Gene Expression Analysis of Grape Plant Response to Xylella fastidiosa. Proceedings of the 2005 Pierce's Disease Research Symposium. p.43-46.

Interpretive Summary: A set of 1,942 genes uniquely expressed in response to Xylella fastidiosa (Xf) infection were cloned from susceptible and resistant sibling genotypes from a V. rupestris x V. arizonica genetic mapping population. The majority (54 %) of these genes were identified for the first time and the rest included genes known to be involved in defense responses. Gene expression profiling of of 20,020 Vitis transcripts showed a significant variation in responses between the susceptible and resistant genotypes to Xf infection. Out of the 793 genes that showed significant responses to Xf infection, 28.1% (223 genes) were derived from this project.

Technical Abstract: In the present study, a set of 1,942 non-redundant SSH ESTs in response to Xylella fastidiosa (Xf) infection were cloned from susceptible and resistant sibling genotypes from a V. rupestris x V. arizonica genetic mapping population. The majorities (54 %) of these ESTs were novel and the rest included genes known to be involved in defense responses. Transcriptional profiling using a custom high-density (382,900 probes) microarray chip of 20,020 Vitis transcripts showed a significant variation in response between the susceptible and resistant genotypes to Xf infection. Out of the 793 transcripts that showed significant response to Xf infection, 28.1% (223 ESTs) were derived from this project.

Last Modified: 9/21/2014
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