Submitted to: Mycoscience
Publication Type: Review Article
Publication Acceptance Date: January 31, 2006
Publication Date: June 15, 2006
Citation: Kurtzman, C.P. 2006. Yeast species - recognition from gene sequence analyses and other molecular methods. Mycoscience. 47:65-71. Technical Abstract: This review discusses DNA-based methods used for identification of yeasts. Nuclear DNA reassociation was the first quantitative molecular method employed for recognition of yeast species and has provided a baseline for interpretation of other molecular comparisons. Among these, gene sequencing is the most definitive with ribosomal RNA gene sequences providing the preponderance of available data. Multigene analyses that include the sequences of protein encoding genes are being increasingly developed to provide a more definitive resolution of species. A number of rapid identification methods, such as DGGE, TGGE and flow cytometry, which are based on species-specific gene sequences, are available for use in diagnostic laboratories.