Page Banner

United States Department of Agriculture

Agricultural Research Service

Title: Genetic Transformation of Sclerotinia Sclerotiorum Through Agrobacterium Tumefaciens-Mediated Transformation.

Authors
item Wamatu, John - WASHINGTON STATE UNIV
item White, David
item CHEN, WEIDONG
item Wang, Xin - WASHINGTON STATE UNIV

Submitted to: North American Pulse Improvement Association
Publication Type: Abstract Only
Publication Acceptance Date: October 1, 2005
Publication Date: N/A

Technical Abstract: In order to study genetic factors of pathogenicity, insertional mutants of Sclerotinia sclerotiorum were generated using Agrobacterium tumefacience-mediated transformation with both mycelial fragments and ascospores. Transformants were tested for number of insertions by Southern hybridization and for stability of hygromycin B resistance, and are being screened for altered pathogenicity on lentil plants and for production of oxalic acid. The tagged mutations will allow identification of mutated genes.

Last Modified: 9/29/2014
Footer Content Back to Top of Page