Page Banner

United States Department of Agriculture

Agricultural Research Service

Research Project: EVOLUTIONARY ENZYMES AND SEPARATION PROCESSES FOR IMPROVED BIOREFINING OF CROPS AND RESIDUES

Location: Bioproduct Chemistry and Engineering Research

Title: Cloning and Characterization of Cold-Active Xylanase Enzymes

Authors
item Lee, Charles
item Kibblewhite, Rena
item Wagschal, Kurt
item Robertson, George
item Wong, Dominic

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: December 16, 2005
Publication Date: April 30, 2006
Citation: Lee, C.C., Accinelli, R., Wagschal, K.C., Robertson, G.H., Wong, D. 2006. Cloning and characterization of cold-active xylanase enzymes. 28th Symposium on Biotechnology for Fuels and Chemicals. Paper No. 1A-41.

Technical Abstract: There is great interest in lignocellulolytic enzymes due to the wide variety of industrial applications for these reagents. At the USDA Agricultural Research Service, we are screening DNA libraries to discover genes encoding new hemicellulolytic enzymes. The libraries were generated both from pure cultures as well as from mixed environmental populations (metagenome). Among the new enzymes isolated are cold-active xylanase enzymes that are categorized as glycosyl hydrolase family 8 and family 10 members. These enzymes have potential applications in processes where the products are heat-sensitive. We describe the cloning, purification, and biochemical characterization of these enzymes.

Last Modified: 7/25/2014
Footer Content Back to Top of Page