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United States Department of Agriculture

Agricultural Research Service

Title: Neospora Caninum: Antibodies Directed Against Tachyzoite Surface Protein Ncsrs2 Inhibit Parasite Attachment and Invasion of Placental Trophoblasts in Vitro

Authors
item Haldorson, G - WSU
item Stanton, J - WSU
item Mathison, B - WSU
item Suarez, Carlos
item Baszler, T - WSU

Submitted to: Experimental Parasitology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: November 7, 2005
Publication Date: March 3, 2006
Citation: Haldorson, G., Stanton, J., Mathison, B.A., Suarez, C.E., Baszler, T. 2006. Neospora caninum: antibodies directed against tachyzoite surface protein ncsrs2 inhibit parasite attachment and invasion of placental trophoblasts in vitro. Experimental Parasitology. 112(3):172-178.

Interpretive Summary: Polyclonal and monoclonal antibodies to native Neospora caninum tachyzoite surface protein NcSRS2 were generated. Both monoclonal and polyclonal antibodies were able to neutralize invasion of Vero host cells and a newly cloned, immortalized ovine trophoblast cell line obtained from primary cultures of ovine placenta. The findings provide rationale to investigate a role for antibodies to NcSRS2 in prevention of N. caninum transplacental transmission in vivo.

Technical Abstract: Polyclonal and monoclonal antibodies to native Neospora caninum tachyzoite surface protein NcSRS2 were generated and tested in vitro for their ability to neutralize tachyzoite attachment to and invasion of host cells. Host cells included Vero cells and a newly cloned, immortalized ovine trophoblast cell line obtained from primary cultures of ovine placenta. The ovine trophoblast had morphology consistent with fetal trophoblasts and expressed mRNA for interferon-tau, a marker for trophoblasts. Native NcSRS2 was used to immunize mice to obtain monospecific anti-NcSRS2 polyclonal serum and anti-NcSRS2 monoclonal antibodies. Compared to irrelevant antibodies, monospecific anti-NcSRS2 serum and two anti-NcSRS2 monoclonal antibodies, 100.2.4.4 and 119.4.9.10, significantly blocked invasion of tachyzoites into both trophoblasts and Vero cells. Parasite attachment, assessed by IFA, was significantly reduced by anti-NcSRS2 mAb 100.2.4.4 and monospecific serum. The findings provide rationale to investigate a role for antibodies to NcSRS2 in prevention of N. caninum transplacental transmission in vivo.

Last Modified: 12/18/2014
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