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United States Department of Agriculture

Agricultural Research Service

Title: Simultaneous Determination of Fluoroquinolones and Tetracyclines in Catfish Muscle Using Hplc-Fluorescence

Authors
item Schneider, Marilyn
item Darwish, Ahmed
item Freeman, Donald

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: February 24, 2006
Publication Date: May 16, 2006
Citation: Schneider, M.J., Darwish, A.M., Freeman, D.W. 2006. Simultaneous determination of fluoroquinolones and tetracyclines in catfish muscle using hplc-fluorescence. Meeting Abstract.

Technical Abstract: Efficient methods are needed for analysis of veterinary drug residues in food. Quite a number of methods are available for single analytes. Multiresidue methods are now increasingly available. It is still rare, however, to find non-mass spectrometric methods which allow for analysis of more than one class of drug residue. An efficient multiresidue method for the simultaneous determination of fluoroquinolones and tetracyclines in catfish muscle has now been developed. This method involves an extraction of the analytes with a mixture of acetonitrile and malonate buffer containing magnesium chloride. After centrifugation and evaporation of the supernatants, the residues are determined using HPLC with fluorescence detection. With this method, five fluoroquinolones and three tetracyclines were determined in fortified catfish muscle at levels of 20, 50, and 100 ng/g. Average recoveries for ciprofloxacin, sarafloxacin, danofloxacin, enrofloxacin, difloxacin, oxytetracycline, tetracycline, and chlortetracycline were in the range of ~60-92% with good RSDs. The limits of quantitation ranged from 0.15 to 1.5 ng/g. Utilization of the method in additional experiments with incurred catfish muscle samples will be described.

Last Modified: 4/17/2014
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