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United States Department of Agriculture

Agricultural Research Service

Title: A Cytogenetic Map of Maize in Oats with Sorghum Bac Fish Probes

Authors
item Bass, Hank - FLORIDA STATE UNIVERSITY
item Figueroa, Debbie - FLORIDA STATE UNIVERSITY
item Amarillo, Ina - FLORIDA STATE UNIVERSITY
item Ring, Brian - FLORIDA STATE UNIVERSITY
item Seigfried, Trent
item LAWRENCE, CAROLYN

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: September 8, 2005
Publication Date: September 8, 2005
Citation: Bass, H.W., Figueroa, D., Amarillo, I., Ring, B.C., Seigfried, T.E., Lawrence, C.J. 2005. A cytogenetic map of maize in oats with sorghum BAC FISH probes [abstract]. 2005 Program Book of the NSF Plant Genome Research Annual Awardee Meeting. Abstract number 0321639.

Technical Abstract: Maize is a major crop in the U.S. and a classical genetic system, particularly well suited for cytogenetics. Its genome is divided among 10 chromosomes, and gene locations can be charted in one of three fundamentally different ways: analysis of trait linkage for a genetic map, DNA sequence determination for a physical map, and microscopic visualization for a cytogenetic map. Integration of the three map types in fruit flies and humans has proven to accelerate genetic research. The production of a similar trio of integrated maps for maize would advance understanding of the genetic material of one of the world's most important cereal crops. The rapid and cost-efficient method this project will employ is to use segments of sorghum DNA (maize-marker-selected sorghum BAC clones) as probes to stain the corresponding regions on maize chromosomes by fluorescence in situ hybridization (FISH). The results will be distributed through GenBank and MaizeGDB. The project will aid in physical map assembly, establish new reagents for chromosome research, and provide insights into the evolution of maize and related grasses.

Last Modified: 9/10/2014
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