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Title: CHARACTERIZATION OF SALMONELLA SPP. ISOLATED FROM PRE- AND POST-CHILL WHOLE BROILER CARCASSES

Author
item PARVEEN, SALINA - UMES
item TAABODI, MARYAM - UMES
item MOHAMMED, TAJ - UMES
item SCHWARZ, JURGEN - UMES
item WHITE, DAVID - FDA
item Oscar, Thomas

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 1/8/2006
Publication Date: 8/13/2006
Citation: Parveen, S., Taabodi, M., Mohammed, T., Schwarz, J., White, D., Oscar, T.P. 2006. Characterization of salmonella spp. isolated from pre- and post-chill whole broiler carcasses. [Abstract] International Association for Food Protection's Annual Meeting. P4-47. pg. 168.

Interpretive Summary:

Technical Abstract: While several reports exist on characterization of Salmonella spp. isolated from processed poultry by phenotypic and genotypic methods, little is known about the characterization of Salmonella spp. isolated from pre- and post-chill carcasses by these methods. The objectives of this study were to characterize Salmonella spp. isolated from pre- and post-chill whole broiler carcasses by serotyping and antimicrobial susceptibility testing, and ascertain the presence of class 1 intergrons and blaCMY genes among isolates of predominant serotypes. A total of 414 Salmonella spp. isolated from pre- and post- chill carcasses were tested for serotyping by standard method. Thirteen different serotypes were recovered from pre- and post-chill carcasses and most prevalent serotypes were Salmonella Kentucky (51.4%; 67.3%) and S. Typhimurium (20.7%; 15.3%). Antimicrobial minimal inhibitory concentrations for all isolates were determined by using the Sensititre system. A total of 76.1% and 83.2% of pre- and post-chill isolates were resistant to one or more antimicrobial agents tested. Approximately 50% of pre- and 43.1% of post-chill isolates were resistant to five or more antimicrobials. Resistance to tetracycline was the most common and observed in 69.3% and 78.7% of pre- and post-chill isolates. Serotypes Kentucky and Typhimurium isolates resistant to sulfisoxazole and ceftiofur were tested for class 1 integrons and blaCMY genes by PCR. Sixty seven percent of the Kentucky possessed blaCMY gene. In contrast, none of the isolates belonging to Kentucky possessed class 1 integrons. However, 30% of serotype Typhimurium possessed class 1 integrons. The blaCMY gene was not detected in serotype Typhimurium. The results of this study indicate that a large number of Salmonella spp. isolated from whole broiler carcasses were resistant to commonly used antibiotics. This research also suggests that class 1 integrons and blaCMY genes contribute to antimicrobial resistance among Salmonella spp.