Author
 |
Niedz, Randall |
|
Evens, Terence |
|
Submitted to: In Vitro Biology Meeting
Publication Type: Abstract Only Publication Acceptance Date: 2/15/2006 Publication Date: 6/3/2006 Citation: Niedz, R.P., Evens, T.J. 2006. Regulating plant tissue growth by mineral nutrition. In Vitro Biology. Interpretive Summary: The basic components for culturing plant tissues in vitro are the salts that provide mineral nutrition to the plant cells. By altering the types and concentrations of the salts used, plant cell growth in vitro will vary dramatically. Determining how plant cells grow over a wide range of salt types and concentrations allows the tissue culturist to grow the cells at whatever rate is required by simply changing the salt formulation used. Technical Abstract: The mineral nutrient requirements of nonembryogenic citrus callus were characterized by breaking the MS salts into the following five factors – NH4NO3, KNO3, Ca-Mg-Cl-Mn-SO4-PO4, metals, and Fe-EDTA. A D-optimal response surface experimental design where each factor was varied over a range of concentrations was constructed. Callus was grown on each treatment combination, fresh/dry weights and friability were measured, and each measured response analyzed by ANOVA. Callus growth ranged from 31% - 135% MS levels and the resulting polynomial model had an R2 of 0.98 and a predictive R2 of 0.92. The model was validated by generating predictions of salt combinations not included in the original design but within the original experimental design space. The responses of callus grown on these new salt combinations were then compared to the predicted values. The implications of this approach in defining the appropriate types and concentrations of mineral nutrients for in vitro responses, including the importance of mineral nutrition, the limitations of traditional methods of defining mineral nutrient formulations, and what it means to “optimize” in vitro responses will be discussed. |
