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United States Department of Agriculture

Agricultural Research Service

Title: Biocontrol Activity of Recombinant Pichia Pastoris Constitutively Expressing Pea Defensin Against Blue Mold of Apple

item Janisiewicz, Wojciech
item Pereira, I - UFRJ, BRAZIL
item Almeida, M - UFRJ, BRAZIL
item Roberts, Daniel
item Wisniewski, Michael
item Kurtenbach, E - UFRJ, BRAZIL

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: May 18, 2006
Publication Date: July 7, 2006
Citation: Janisiewicz, W.J., Pereira, I.B., Almeida, M.S., Roberts, D.P., Wisniewski, M.E., Kurtenbach, E. 2006. Biocontrol activity of recombinant Pichia pastoris constitutively expressing pea defensin against blue mold of apple. Phytopathology. 96:S53.

Technical Abstract: A peptide (Psd1) isolated from pea seeds has been shown to have strong antifungal activity against a variety of fungi. The gene for this peptide was identified, and a recombinant peptide (46 residues) with activity similar to the original one was produced. We used a binary vector pGAPZalphaC containing this gene under a constitutive GAP promoter to transform Pichia pastoris strains X 33 and GS 115. Both strains transformed well and expressed the rPsd1 peptide. Untransformed strain X 33 grew better than GS 115 in Golden Delicious apple wounds, so biocontrol tests focused on X 33 transformants. The incidence of blue mold decay caused by Penicillium expansum on apples treated with the fungus alone or in combination with the parent strain, a transformant with the empty pGAPZaC vector or a transformant with the plasmid containing rPsd1 gene was 100%, 56%, 33%, and 11%, respectively, after 5 days incubation at 24 C. The consistency of this control still must be established.

Last Modified: 4/22/2015
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