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United States Department of Agriculture

Agricultural Research Service

Title: Growth of Campylobacter on Media Supplemented with Organic Acids

Author
item Hinton, Jr, Arthur

Submitted to: Poultry Science Association Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: April 30, 2006
Publication Date: July 16, 2006
Citation: Hinton Jr, A. 2006. Growth of campylobacter on media supplemented with organic acids [abstract]. Poultry Science Association Meeting Abstract. 85(Suppl.1):132.

Technical Abstract: Campylobacter spp. are the main cause of bacterial foodborne illnesses in humans, and contaminated poultry products are major sources of campylobacteriosis. In this study, the growth of Campylobacter spp. in media supplemented with organic acids was examined. Tryptose-yeast extract basal broth medium was supplemented with 0, 10, 20, 30, 40, or 50 mM of citric, fumaric, lactic, malic, or succinic acid then inoculated with cultures of Campylobacter coli ATCC 33559, Campylobacter fetus subsp. fetus ATCC 27349, or Campylobacter jejuni subsp. jejuni ATCC 33560. Inoculated media were transferred into a MACS VA500 Microaerophilic Workstation, and 0.1 ml of the bacterial suspensions were dispensed into wells of a Honeycomb 2 cuvette plate. Each cuvette well was overlaid with 0.1 ml of sterile mineral oil, and the filled Honeycomb 2 plates were transferred to the incubator tray of a Bioscreen C Microbiology Reader. The microbiology reader measured the absorbance of the cultures after cultures were incubated at 37oC for 48 h. Results indicated that growth of C. coli ATCC 33559 and C. jejuni subsp. jejuni ATCC 33560 were significantly (P < 0.05) higher when cultured in media supplemented with 20-50 mM of either organic acid than when cultured in media not supplemented with an organic acid. Growth of C. fetus subsp. fetus ATCC 27349 was significantly (P < 0.05) greater in media supplemented with 10 to 50 mM of either organic acid, except for citric acid, than in non-supplemented media. Growth of the Campylobacter isolates was also measured in basal media supplemented with a mixture of 10, 20, 30, 40 or 50 mM of fumaric, malic, lactic, and succinic acids utilizing the same methods used to examine growth in media supplemented with individual organic acids. Results indicated that the growth of all isolates was significantly (P < 0.05) greater in media supplemented with mixtures containing 10 to 40 mM of each organic acid than in non-supplemented media. Findings of this study illustrate that growth of Campylobacter spp. may be enhanced when the bacteria are cultured in media supplemented with selected organic acids. These supplemented media may be useful in conducting research on intervention methods for reducing contamination of poultry products by this pathogen. Keywords: Campylobacter, media, organic acids

Last Modified: 10/21/2014
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