ARS | Publication request: MOLECULAR CHARACTERIZATION OF THE CORE SUBSET OF THE US PEANUT GERMPLASM CORE COLLECTION USING SSR MARKERS

Submitted to: American Peanut Research and Education Society Abstracts
Publication Type: Proceedings
Publication Acceptance Date: April 16, 2006
Publication Date: July 14, 2006
Citation: Kottapalli, K.R., Burow, G.B., Burke, J.J., Puppala, N., Burow, M.D. 2006. Molecular characterization of the core subset of the us peanut germplasm core collection using ssr markers. American Peanut Research and Education Society. Savannah, Georgia. July 11-14, 2006.

Technical Abstract: Genetic improvement of peanut is hampered by limited genetic variability in the germplasm used commonly by breeding programs. Greater variability is present in the peanut core and core subset collections, but utilization of these collections could be enhanced by genetic characterization of these collections. We report characterization of the genetic characterization of the core subset of the peanut core collection using simple sequence repeat (SSR) markers. Seventy two peanut accessions appearing uniform by visual measurement in the field were genotyped using 73 primer pairs (twelve of these markers were mapped SSR loci) and scored for the presence or absence of amplified bands. Based on phylogenetic and phenetic studies employing a combination of clustering and parsimony methodologies, the four market types grown in the U. S. were grouped. Substantial genetic variation was found to exist in the core subset, contrary to previous reports of little or no variation in the cultivated species. A group of twelve unlinked markers with known map positions identified lower variation among the accessions but was found sufficient to identify both botanical and market types and gave results similar to those obtained using the larger number of primer pairs. The genetic variation observed indicate that SSR markers are highly suitable for development of genetic maps of cultivated tetraploid peanut.