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United States Department of Agriculture

Agricultural Research Service

Title: High Throughput Protein Identification Using 2-Dimensional Difference Gel Electrophoresis and Robotic Spot Picking for Aluminum Tolerance-Related Maize Root Tip Proteins

Authors
item Thannhauser, Theodore
item Yang, Yong
item Zhang, S. - CORNELL UNIVERSITY
item Sherwood, Robert - CORNELL UNIVERSITY
item Isaac, Issa - GENOMIC SOLUTIONS INC.

Submitted to: Journal of American Society for Mass Spectrometry
Publication Type: Abstract Only
Publication Acceptance Date: June 13, 2006
Publication Date: August 1, 2006
Citation: Thannhauser, T.W., Yang, Y., Zhang, S., Sherwood, R., Isaac, I. 2006. High throughput protein identification using 2-dimensional difference gel electrophoresis and robotic spot picking for aluminum tolerance-related maize root tip proteins. Journal of American Society for Mass Spectrometry. 17:46.

Technical Abstract: Two-dimensional difference gel electrophoresis (2-D DIGE) is the most effective method utilized to carry out gel-based quantitative proteomics. Unfortunately, the most popular image analysis software used to process DIGE images (DeCyder) produces picking coordinates in a format that is incompatible with most commercially available spot picking robots. To achieve the full promise of proteomic research it is necessary to perform protein identification experiments on such a scale that automation is required. Thus, there is a need for transformation software to convert the DeCyder generated picking coordinates to a format compatible with commonly available robots. Here we present a protocol using 2-D DIGE, DeCyder and the ProPic spot picking robot incorporating such a program that greatly facilitates protein identification.

Last Modified: 11/28/2014
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