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United States Department of Agriculture

Agricultural Research Service

Title: Reduction of Incubation Times for Viral Pathogen Immunoassays by Using Elevated Temperatures As Monitored by Atomic Force Microscopy

Authors
item Kwarta, Karen - IOWA STATE UNIVERSITY
item Driskell, Jeremy - IOWA STATE UNIVERSITY
item Ridpath, Julia
item Neill, John
item Porter, Marc - IOWA STATE UNIVERSITY

Submitted to: American Chemical Society National Meeting
Publication Type: Abstract Only
Publication Acceptance Date: September 10, 2006
Publication Date: September 10, 2006
Citation: Kwarta, K.M., Driskell, J.D., Ridpath, J.F., Neill, J.D., Porter, M.D. 2006. Reduction of Incubation Times for Viral Pathogen Immunoassays by Using Elevated Temperatures as Monitored by Atomic Force Microscopy [abstract]. American Chemical Society. Paper No. 993506.

Technical Abstract: This presentation describes the use of elevated temperatures to reduce the time needed to selectively extract viruses from a sample solution onto a solid capture substrate coated with monoclonal antibodies (Mab) specific for the viruses. Raising incubation temperatures causes a change in the antibody-virus interaction and in the rate of mass transport for the virus to the substrate. Porcine parvovirus (PPV) and feline calicivirus (FCV) were chosen as model targets because their well defined size and shape enables facile enumeration by imaging with atomic force microscopy (AFM). This readout approach yielded a label-free assay, which was used to study binding kinetics. The binding kinetics of both viruses to their respective Mab's was examined at two different temperatures (25 C and 37 C). Dose-response curves show that the extraction time can be reduced to 1 h at 37 C while only reducing the detection limit achieved with 16 h incubation at 25 C by a factor of two.

Last Modified: 9/20/2014
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