|Liu, Zhenyu - UNIV OF WISC - MADISON|
Submitted to: Plant and Animal Genome Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: October 6, 2005
Publication Date: January 14, 2006
Citation: Halterman, D.A., Liu, Z. 2006. Identification of RB-orthologous genes from late blight resistant diploid potato [abstract]. Plant and Animal Genome Conference Proceedings. p 118. Technical Abstract: Late blight, caused by the oomycete pathogen, Phytophthora infestans, is a devastating disease of potatoes and tomatoes. A gene RB, cloned from the Mexican diploid potato species Solanum bulbocastanum, confers broad spectrum resistance to potato late blight. To identify RB-like genes and understand the molecular basis of RB-mediated late blight resistance, we have amplified RB orthologs from other late-blight resistant potato species using a homology-based PCR approach. Comparison of RB-like genes from resistant and susceptible accessions have revealed interesting similarities and differences that may provide insights into gene function. Transcription of these genes has been tested using RT-PCR. Transcribed RB orthologs encoding full-length proteins will be tested for function through stable transformation into a susceptible potato cultivar.