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United States Department of Agriculture

Agricultural Research Service

Research Project: DEVELOPMENT AND EVALUATION OF IMPROVED MATERIALS FOR MYCOTOXIN ANALYSIS

Location: Bacterial Foodborne Pathogens & Mycology Research Unit

Title: Recent Developments in Trichothecene Analysis

Authors
item Pascale, Michelangelo - INST. OF SCIENCES OF FOOD
item Lippolis, Vincenzo - INST. OF SCIENCES OF FOOD
item Maragos, Chris
item Viscontini, Angelo - INST. OF SCIENCES OF FOOD

Submitted to: American Chemical Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: May 1, 2006
Publication Date: September 10, 2006
Citation: Pascale, M., Lippolis, V., Maragos, C., Viscontin, A. 2006. Recent developments in trichothecene analysis [abstract]. American Chemical Society. AGFD No. 206.

Technical Abstract: Gas chromatographic (GC) methods based on ECD, FID or MS detection are the most widely used methods for quantitative determination of the more toxic type-A trichothecenes (T-2 and HT-2 toxins). Deoxynivalenol (DON), the main type-B trichothecene frequently occurring in cereal grains, is commonly detected by HPLC/UV at ppb levels with good accuracy and precision. Recently, we have developed a method for the simultaneous determination of T-2 and HT-2 toxins in cereal grains using immunoaffinity column clean-up, labeling with 1-anthroylnitrile, and HPLC with fluorescence detection (FD). Moreover, a fluorescence polarization immunoassay (FPIA) has been used for rapid quantification of DON in wheat. Ongoing research efforts focus on improvement of the HPLC/FD method for T-2 and HT-2 using new labeling reagents (i.e. 1-naphthoyl chloride, 2-naphthoyl chloride, pyrene-1-carbonyl cyanide), and the optimization of FPIA for rapid screening of DON in soft and durum wheat kernels, semolina and pasta.

Last Modified: 9/2/2014
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