ARS | Publication request: Single Laboratory Validation of a LC/UV/MS Method for Determination of Multiple Water Soluble Vitamins in Dietary Supplements (AOAC Annual Meeting, Minneapolis, MN, Sept, 2006)

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: May 1, 2007
Publication Date: September 15, 2007
Citation: Chen, P., Wolf, W.R. 2007. Single Laboratory Validation of a LC/UV/MS Method for Determination of Multiple Water Soluble Vitamins in Dietary Supplements. AOAC Annual Meeting, September 15, 2006, Minneapolis, Minnesota.

Technical Abstract: Today there is increased interest in accurately assessing the total dietary intake of vitamins from all sources, including foods and dietary supplements. A dietary supplement ingredient database comprising analytical values is under joint development by the ARS and the ODS/NIH. While current “official” microbiological assay methods for water soluble vitamins are highly sensitive, they involve detailed laborious procedures that are time-consuming and require multiple determinations to achieve required precision. These microbiological procedures were developed over 50 years ago and are generally outdated in light of new technology. While there are reports in the literature of Liquid Chromatography (LC) methods for the determination of water soluble vitamins in foods, these methods have not been rigorously validated for general use in response to food labeling regulatory requirements, and often targeted only one vitamin at a time. We have investigated a LC/UV/MS method using a C18 reverse phase column and Quattro Micro triple-quad mass spectrometer for simultaneous determination of multiple water soluble vitamins (B1 - thiamine, C - ascorbic acid, B6 - pyridoxine, nicotinamide, pantothenic acid, and B2 - riboflavin). The method is specifically designed to quantify water-soluble vitamins in dietary supplements that contain very different levels of different water-soluble vitamins. This method is being applied initially to determine these vitamins in the new multi-vitamin SRM, being jointly developed with NIST and ODS/NIH. Single Laboratory Validation of the method has investigated the parameters of extraction procedure, limits of quantitation (LOQ), dynamic range, accuracy, precision, and ruggedness.