Title: Investigation of imprinted polymers for the selective solid phase extraction of moniliformin Authors
Submitted to: World Mycotoxin Forum, the Third Conference
Publication Type: Abstract Only
Publication Acceptance Date: September 21, 2006
Publication Date: November 6, 2006
Citation: Appell, M.D., Maragos, C.M., Kendra, D.F. 2006. Investigation of imprinted polymers for the selective solid phase extraction of moniliformin [abstract]. World Mycotoxin Forum, the Fourth Conference. p. 109. Technical Abstract: Moniliformin is a toxic metabolite produced by several Fusarium species, such as proliferatum and subglutinans. This low molecular weight mycotoxin is generally isolated as the sodium or potassium salt of semisquaric acid. Studies have found concentrations of moniliformin to be associated with adverse effects in a variety of animals, including rats and poultry. A reported mode of action of moniliformin is the inhibition of pyruvate dehydrogenase, resulting in decreased mitochondrial respiration through the disruption of the Krebs cycle. Current methods for moniliformin detection include the use of conventional analytical techniques such as HPLC-UV and mass spectroscopy. In addition, a method has been developed using capillary zone electrophoresis diode array detection. With simple derivatization of moniliformin, fluorescence and gas chromatography can be accomplished. Several procedures have been developed for moniliformin extraction and clean-up. Extraction has been reported with various concentrations of acetonitrile/water and with ion pair reagents. Extracts are typically put through clean-up steps which include the use of C18 or SAX columns. A moniliformin selective binding material can assist in the clean-up and the determination of moniliformin levels. Molecularly imprinted polymers (MIPs) are materials that can be designed and optimized for selectivity. Imprinted polymers that exhibit selective moniliformin binding compared to non-imprinted polymers have been characterized by binding assays using HPLC-UV to detect moniliformin levels. Moniliformin binding MIPs were prepared with moniliformin analogs as templates and optimal polymer compositions were dependent on the imprint molecule. Evaluation of these materials in molecularly imprinted solid phase extraction (MISPE) columns identified several imprinted polymers possessing selective moniliformin binding compared to non-imprinted polymers in reported extraction solvents. In addition, bound moniliformin was eluted from the MISPE columns with the LC-mobile phase. The imprinted polymer was capable of the preconcentration and clean-up of moniliformin from moniliformin spiked corn extracts. These materials show potential to assist in the determination of moniliformin levels in corn.