Technical Abstract: The chemostat model has been an important tool in studying intestinal microflora. To date, several competitive exclusion products have been developed from such studies as prophylactic treatment against pathogenic bacteria. A continuous flow chemostat model of a feral pig was developed using inocula from the cecal contents of a wild boar caught in East Texas. Several strains of antibiotic-sensitive bacteria were isolated including Bacteroides, Lactobacillus, Enterococcus and Clostridium sp. This study reports on the characterization of a multidrug-resistant Clostridum hathewayi strain that was isolated from this feral pig's cecal contents maintained in a continuous flow chemostat system showing high resistance to carbapenems and macrolides (including the growth promoter tylosin) Clostridium hathewayi has been documented to be pathogenic to both humans and animals. Feral pigs may be a potential source of antibiotic resistant strains and may pose potential risk to domestic species. Characterizing the intestinal microflora and antibiotic resistant strains found in feral animals will allow us to elucidate its prevalence and pathogenicity in potential sources of resistant pools of bacteria.