ARS | Publication request: Validation of a LC/UV/MS Method for Determination of Multiple Water Soluble Vitamins in Dietary Supplements (Experimental Biology, April, 2007, Washington, D.C.)

Submitted to: Experimental Biology
Publication Type: Abstract Only
Publication Acceptance Date: November 3, 2006
Publication Date: April 1, 2007
Citation: Wolf, W.R., Chen, P. 2007. Validation of a LC/UV/MS Method for Determination of Multiple Water Soluble Vitamins in Dietary Supplements. Experimental Biology, April 25, 2007, Washington, D.C.

Technical Abstract: There is increased interest in accurately assessing the total dietary intake of vitamins from all sources, including foods and dietary supplements. A Dietary Supplement Ingredient Database comprising analytical values is under joint development by the ARS and the ODS/NIH. While current "official" microbiological assay methods for water soluble vitamins (WSV), originally developed over 50 years ago, are highly sensitive, they involve detailed laborious procedures and require multiple determinations to achieve required precision. While there are reports in the literature of Liquid Chromatography (LC) methods for the determination of WSV in foods, these methods have not been rigorously validated for general use, and often target only one vitamin at a time. We have investigated a LC/UV/MS method using a C18 reverse phase column and dual detection with a diode array(UV)and a triple-quad mass spectrometer for simultaneous determination of multiple water soluble vitamins (B1-thiamine, vitamin C, B6-pyridoxine, nicotinamide, pantothenic acid, biotin and B2-riboflavin). The method is specifically designed to quantify water-soluble vitamins in dietary supplements that contain very different levels of vitamins. Single Laboratory Validation of the method has investigated the parameters of extraction procedure, limits of quantitation (LOQ), dynamic range, accuracy, precision, and ruggedness.