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United States Department of Agriculture

Agricultural Research Service

Research Project: DETECTION, SOURCE IDENTIFICATION, ENVIRONMENTAL TRANSPORT, FATE, AND TREATMENT OF PATHOGENIC MICROORGANISMS DERIVED FROM ANIMAL WASTES Title: Fate of E. coli O157:H7 in the Environment

Author
item Ibekwe, Abasiofiok

Submitted to: Soil Science Society of America Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: October 20, 2006
Publication Date: November 12, 2006
Citation: Ibekwe, A.M. 2006. Fate of E. coli O157:H7 in the Environment. Soil Science Society of America Annual Meeting in Indianapolis, IN Nov 12-16, 2006. Paper No. 88-4

Technical Abstract: The safety of land application of manure and the potential for food and water contamination by pathogens from agricultural runoffs have been the concern of many groups throughout the world. Their origin has been both point and non-point sources to most watersheds. Their fate and transport in watersheds supplying drinking water, from their deposit in feces and manure originating from Concentrated Animal Operations to their dispersion in major creeks is poorly understood. Multiplex fluorogenic PCR was used in conjunction with plate counts to quantify fate of E. coli O157:H7 in soil, manure, feces, and dairy washwater. The death of the majority of E. coli O157:H7 in soil takes place as a fast exponential decay. After a few weeks, the initial purely exponential decay of the killing curve changes to a more complex, slowly decreasing function. After 60 days a small fraction of the cells survived, and this fraction may be important factor in re-contamination of the environment or re-infection of cattle.

Last Modified: 9/22/2014
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