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United States Department of Agriculture

Agricultural Research Service

Research Project: AFLATOXIN CONTROL THROUGH TARGETING MECHANISMS GOVERNING AFLATOXIN BIOSYNTHESIS IN CORN AND COTTONSEED

Location: Food and Feed Safety Research

Title: Comparative and Functional Genomics in Identifying Aflatoxin Biosynthetic Genes

Authors
item Yu, Jiujiang
item Wilkinson, Jeffery - MS STATE UNIV
item Payne, Gary - NCSU RALEIGH
item Machida, Masayuki - AIST JAPAN
item Campbell, Bruce
item Bennett, Joan - RUTGERS UNIV
item Bhatnagar, Deepak
item Cleveland, Thomas
item Nierman, William - TIGR ROCKVILLE MD

Submitted to: Fungal Genetics Conference/Asilomar
Publication Type: Abstract Only
Publication Acceptance Date: January 30, 2007
Publication Date: March 18, 2007
Citation: Yu, J., Wilkinson, J., Payne, G., Machida, M., Campbell, B.C., Bennett, J., Bhatnagar, D., Cleveland, T.E., Nierman, W. 2007. Comparative and Functional Genomics in Identifying Aflatoxin Biosynthetic Genes. In: Proceedings of the 24th Fungal Genetics Conference and the 4th International Aspergillus Meeting, March 18-25, 2007, Asilomar, CA. p. 82.

Technical Abstract: Identification of genes involved in aflatoxin biosynthesis through Aspergillus flavus genomics has been actively pursued. A. flavus Expressed Sequence Tags (EST’s) and whole genome sequencing have been completed. Groups of genes that are potentially involved in aflatoxin production have been profiled using microarrays under different culture conditions and during fungal infection of corn. Preliminary annotation of the sequence revealed that there are about 12,000 genes in the A. flavus genome. Many genes in the genome, which potentially encode for enzymes involved in secondary metabolite production, such as polyketide synthases, non-ribosomal peptide synthases and cytochrome P450 monooxigenases, have been identified. Comparative analysis of A. flavus genome with food grade industrial fermentation organism A. oryzae can help understanding the mechanism of aflatoxin biosynthesis and solving the problem of aflatoxin contamination.

Last Modified: 9/20/2014
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