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United States Department of Agriculture

Agricultural Research Service

Research Project: NOVEL TECHNOLOGIES AND TECHNIQUES FOR THE DETECTION OF RESIDUES, TOXINS, AND OTHER CHEMICALS IN FOODS Title: Multiresidue Determination of Fluoroquinolone Antibiotics in Chicken Serum and Muscle using Liquid Chromatography-Fluorescence-Mass Spectrometryn

Authors
item Schneider, Marilyn
item Reyes-Herrera, Ixchel - UNIV OF ARKANSAS
item Donoghue, Dan - UNIV OF ARKANSAS

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: May 16, 2007
Publication Date: June 10, 2007
Citation: Schneider, M.J., Reyes-Herrera, I., Donoghue, D.J. 2007. Multiresidue Determination of Fluoroquinolone Antibiotics in Chicken Serum and Muscle using Liquid Chromatography-Fluorescence-Mass Spectrometryn. Meeting Abstract.

Technical Abstract: A multiresidue method has been developed for determination of fluoroquinolone (FQ) antibiotics in chicken serum and muscle. FQs are extracted from either serum or muscle with acetonitrile/ammonium hydroxide and the extracts are defatted, evaporated and resuspended in buffer, then filtered and analyzed by liquid chromatography-fluorescence-mass spectrometryn, achieving simultaneous quantitation (fluorescence) and confirmation (MSn). Eight FQs were determined in chicken serum and muscle samples fortified at 10-100 ng/g. Recoveries of 68-90% were obtained for muscle, and 71-99% for serum, with relative standard deviations <10%. Low limits of quantitation were achieved for both muscle and serum (less than or equal to 5 ng/g). Confirmation was accomplished by comparison of MS2 or MS3 product ion ratios to those of standard FQs. As a further component of this study, chickens were dosed with enrofloxacin for three days at a concentration of 0.050 ng/mL in drinking water, and both muscle and serum samples were analyzed. The results show that enrofloxacin can be determined in both serum and muscle of these chickens for up to at least 48 hr after withdrawal from dosing, and suggest that serum can provide an efficient means for monitoring FQ levels in chicken.

Last Modified: 12/17/2014
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