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United States Department of Agriculture

Agricultural Research Service

Research Project: AUGMENTATIVE BIOLOGICAL CONTROL AND MASS REARING FOR BENEFICIAL AND PEST INSECTS

Location: Biological Control of Pests Research Unit

Title: Sequences of cDNA Clones from Lygus lineolaris (Palisot de Beauvois) (Heteroptera: Miridae)

Author
item Allen, Margaret

Submitted to: Genbank
Publication Type: Other
Publication Acceptance Date: April 9, 2007
Publication Date: May 13, 2007
Citation: Allen, M. L. 2007. Sequences cDNA Clones from Lygus lineolaris (Palisot de Beauvois) (Heteroptera: Miridae). Genbank.

Interpretive Summary: The tarnished plant bug, Lygus lineolaris, is an important pest of many crops including cotton, alfalfa, strawberries, seed crops, nuts, and vegetable crops. Plant bug feeding damage results in death of young fruits, leaves, and flowers. Genes identified from this type of insect have been few, limiting development and testing of new gene-based pesticides. These 18 additional sequences, filed in public data repositories, provide scientists with genetic tools to study populations of plant bugs.

Technical Abstract: Eighteen sequences have been deposited to augment the expressed sequences of Lygus lineolaris in the National Center for Biotechnology Information database, GenBank. These sequences were obtained from laboratory reared specimens. Total RNA was extracted from specimens, and then pooled and used to obtain polyadenylated message RNA. This material reverse transcribed and amplified with polyadenylation-specific primers, then size fractionated. Smaller (less than 1,000 nucleotides) sequences were ligated into sequencing vector and transformed into E. coli cells. Colonies were directly amplified with PCR to choose clones for sequencing. Sequences not already included in GenBank, numbered EF541356 through EF541373 were submitted.

Last Modified: 9/22/2014
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