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United States Department of Agriculture

Agricultural Research Service

Research Project: COUNTERMEASURES TO CONTROL AND SUPPORT ERADICATION OF BOVINE VIRAL DIARRHEA VIRUS (BVDV) Title: Interlaboratory comparison of diagnostic testing methods for bovine viral diarrhea virus

Authors
item Huyke, Narda - USDA-APHIS-VS-NVSL, AMES,
item Swenson, Sabrina - USDA-APHIS-VS-NVSL, AMES,
item RIDPATH, JULIA

Submitted to: American Association of Veterinary Laboratory Diagnosticians
Publication Type: Abstract Only
Publication Acceptance Date: July 15, 2007
Publication Date: October 18, 2007
Citation: Huyke, N., Swenson, S.L., Ridpath, J.F. 2007. Interlaboratory comparison of diagnostic testing methods for bovine viral diarrhea virus [abstract]. American Association of Veterinary Laboratory Diagnosticians 50th Annual Conference. p. 62.

Technical Abstract: The NVSL, in collaboration with ARS, developed a proficiency panel for bovine viral diarrhea virus testing. Twenty-eight participants used the panel for virus detection by antigen ELISA, virus isolation, or PCR. The panel consisted of 15 or 16 diluted serum and buffy coat samples from known negative and persistently infected cattle. Four of the samples were deemed negative, and 12 were positive by PCR (expected results). We received 28 results for the antigen ELISA, 21 results for virus isolation, and 25 results for PCR; 10 of the PCR results included virus typing. For the antigen ELISA, 10 of 28 participants identified all samples correctly. For virus isolation, 5 of 21 participants identified all samples correctly. For PCR, 10 of 25 participants identified all samples correctly. Approximately 50% of our samples were identified as expected by all participants across all tests. Laboratories consistently identified negative samples, with 1 false positive by antigen ELISA, 2 false positives from one participant by virus isolation, and no false positive by PCR.

Last Modified: 9/29/2014
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