Submitted to: Molecular Breeding
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: October 18, 2007
Publication Date: February 19, 2008
Citation: Bae, J., Halterman, D.A., Jansky, S.H. 2008. Development of a Molecular Marker Associated with Verticillium Wilt Resistance in Diploid Interspecific Potato Hybrids. Molecular Breeding. 22(1):61-69. Interpretive Summary: Verticillium wilt (VW) is a widespread and serious potato disease caused by the soilborne fungi Verticillium dahliae and V. albo-atrum. Previously, we have identified resistance to VW in some wild potato species. This resistance is currently being integrated into cultivated potato varieties. Here, we have identified a molecular marker associated with resistance to VW in hybrid potato plants. This marker will be a valuable tool for potato breeders that want to integrate resistance into their breeding lines.
Technical Abstract: Verticillium wilt (VW) is a widespread and serious potato (Solanum tuberosum) disease caused by the soilborne fungi Verticillium dahliae and V. albo-atrum. Breeding for VW resistance in potato is challenging due to ambiguous symptom expression, a lack of high throughput screening techniques, and variability in colonization by the fungus among and within plants. Genetic studies have identified major genes that confer resistance in diploid Solanum chacoense (Vc) and interspecific hybrids (Vw and Vt). However, to date, these genes have not been used to develop molecular markers for the identification of resistant clones. Tomato Ve1 and Ve2 gene sequence information was used to amplify candidate Ve gene orthologs from both resistant and susceptible diploid potato hybrids. A CAPS marker was generated to track VW resistance in a backcross population segregating for resistance. The marker was also tested for its usefulness in other breeding lines. Our results indicate that this marker is effective for selection of the Vw gene in segregating breeding populations.