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United States Department of Agriculture

Agricultural Research Service

Title: Generation of expressed sequence tags (ESTs) from cultivated peanuts for gene discovery and marker development

Authors
item Guo, Baozhu
item Chen, Xiaoping - UNIV OF GA
item Dang, Phat
item Holbrook, C
item Culbreath, Albert - UNIV OF GA
item Scully, Brian
item Yu, Jiujiang
item Nierman, William - J.CRAIG VENTER
item Cleveland, Thomas

Submitted to: Aflatoxin Elimination Workshop Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: October 19, 2007
Publication Date: March 5, 2008
Citation: Guo, B., Chen, X., Dang, P.M., Holbrook, Jr., C.C., Culbreath, A., Scully, B.T., Yu, J., Nierman, W.C., Cleveland, T.E. 2007. Generation of expressed sequence tags (ESTs) from cultivated peanuts for gene discovery and marker development [abstract]. Proceedings of the 2007 Annual Multi-crop Aflatoxin/Fumonisin Elimination and Fungal Genomics Workshop, October 22-24, 2007, Atlanta, GA. p. 88.

Technical Abstract: Aflatoxin contamination caused by Aspergillus fungi is a great concern in peanut production worldwide. Pre-harvest Aspergilli infection and aflatoxin contamination are usually severe in peanuts that are grown under drought stressed conditions. Genomic research can provide new tools to study plant-microbio interaction and enhance crop genetic improvement. However, genome research in peanut is far behind other crops due to the shortage of essential genome infrastructure, tools, and resources. The objectives are to develop tools and resources and provide putative genes and EST-based markers for peanut researchers and provide microarray technology for the peanut community to study peanut-Aspergillus interaction and to mitigate aflatoxin contamination in peanut. We have completed sequencing the 5’ ends of total 44,064 clones from 10 cDNA libraries, resulting over 13,824 UniESTs, which will be used for production of peanut long-oligo microarray for the peanut community. These sequence data have been made available to the community in order to develop genomic tools and resources for deciphering the chromosomal location and biological function of genes in the peanut genome and mitigating peanut food safety issues (ES702796-ES717096, ES717097-ES724546, ES751526-ES768453). Our research objectives are two fold: 1) construction of peanut 70-mer oligo microarray in collaboration with Dr. Nierman and Dr. Yu, and 2) development of markers/genes associated with the resistance. Total 8402 oligos of size of 60-80bp in length have been designed for oligo microarray. A panel of 16 diverse peanut genotypes has been screened for genetic diversity, and a mapping population has been developed between Tifrunner and GT-C20 for advancement to RILs (recombinant inbred lines).

Last Modified: 8/22/2014
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