|Barrett-Wilt, Greg - UW MADISON|
Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: November 5, 2007
Publication Date: January 12, 2008
Citation: Halterman, D.A., Barrett-Wilt, G. 2008. Analysis Of The Potato Proteome During The Late Blight Resistance Response Mediated By The Gene RB [abstract]. Plant and Animal Genome Conference. Paper No. P896. Technical Abstract: Late blight, caused by the oomycete pathogen, Phytophthora infestans, is a devastating disease of potatoes and tomatoes. A gene RB, cloned from the Mexican diploid potato species Solanum bulbocastanum, confers robust resistance to potato late blight. RB encodes a CC-NBARC-LRR (coiled coil, nucleotide binding site, leucine-rich repeat) resistance protein. RB is involved in perception of P. infestans, through recognition of the effector ipiO, and signaling to elicit resistance within the plant cell cytoplasm. By studying the proteins involved in the resistance response, we hope to better understand the disease resistance mechanisms mediated by RB. We are using two proteomic approches: 1) complex protein mixtures are separated by two-dimensional electrophoresis (2DE) according to charge and size (molecular weight) and selected peptides are identified by mass spectrometry (MS); 2) quantitative analysis of protein abundance using nitrogen15 (N15) labeling of all plant proteins, followed by analysis using multidimensional protein identification technology (MudPIT). In all experiments, we are comparing proteins isolated from a late blight resistant transgenic potato plant SP951, containing a single copy of RB, with those from the susceptible control Katadhin (Solanum tuberosum cv. Katadhin) at 0, 24, and 72h post-inoculation with P. infestans.