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United States Department of Agriculture

Agricultural Research Service

Research Project: CATFISH GENETICS, BREEDING, AND PHYSIOLOGY Title: Proteomic analysis of head kidney tissue from resistant and susceptible families following challenge with Edwarsiella ictaluri

Authors
item Booth, Natha
item Bourgeois, Lanie

Submitted to: Aquaculture
Publication Type: Abstract Only
Publication Acceptance Date: January 30, 2007
Publication Date: N/A

Technical Abstract: One of the goals of the Catfish Genetics Research Unit is the identification of genetic markers for resistance to viral and bacterial diseases in channel catfish for use in selective breeding. A pilot study was performed to compare proteomic profiles of resistant and susceptible families of channel catfish following a submersion challenge with virulent Edwardsiella ictaluri. Head kidney samples were taken from three fish from each tank at 2 and 6 hours post-immersion. The head kidney samples were pooled from each tank, and total protein was isolated and analyzed by 2-D-gel electrophoresis using a pI range of 4.0 to 7.0. Comparisons were made between proteomic profiles from infected and uninfected resistant and susceptible families. Only proteins expressed in pooled fish from all three replicate tanks were selected for analysis. At 2 hours post-immersion, preliminary results obtained from comparison of peptide fingerprint analysis with NCBI databanks identified a heat shock protein 90-beta showing C. I. % score of 83.696 to that of Danio rerio from susceptible infected families, and a fructose-1,6-bisphosphatase 1-like protein with a score of 99.91% (Danio rerio) from the resistant infected families. At 6 hours post-immersion a RAB-11-like protein with a score of 99.695% (Danio rerio) was identified from the resistant infected families, a tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon with a score of 98.58% (Danio rerio) was identified in the resistant infected and uninfected families, and a glyceraldehyde-3-phosphate dehydrogenase with a score of 99.918%(Danio rerio) was detected in all families and all treatments. While limited, these results demonstrate the potential for use of proteomic techniques in the search for genomic markers for disease resistance in channel catfish.

Last Modified: 10/31/2014
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