Technical Abstract: Food producing animals can be reservoirs of human pathogenic bacteria such as enterohemorrhagic Escherichia coli (O157- and non-O157 Shigatoxin-producing E. coli), Salmonella, and Campylobacter, often harboring these pathogens within their gastrointestinal tracts. Carrier animals colonized by these bacteria often exhibit few if any symptoms which makes their detection by visual examination difficult and therefore their removal prior to slaughter almost impossible. Post harvest technologies such as steam pasteurization, hot water rinses, and organic acid sprays have been implemented to reduce potential contamination of carcasses by these bacteria during slaughter and subsequent handling but none are completely infallible as product recalls and outbreaks of human food-borne disease continue to occur. Interest exists, therefore, in the development of on-farm interventions that can reduce the carriage of zoonotic pathogens before they are presented for slaughter and processing. Concern also exists among public health officials and consumers that continued use of antibiotics to control disease and enhance production is contributing to the emergence and proliferation of antimicrobial-resistant microbial populations. As a consequence, livestock producers are under increasing pressure to reduce their continued use of and dependence on antibiotics. There is an urgent need for research that can deliver alternative on-farm pathogen control strategies that where possible should contribute to the efficiency and profitability of animal production. This chapter presents a review of recent pre-harvest food safety research activities conducted at the Food and Feed Safety Research Unit.