ARS | Publication request: Identification of peanut hybrids using microsatellite markers and horizontal polyacrylamide gel electrophoresis

Submitted to: Peanut Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: May 13, 2008
Publication Date: October 1, 2008
Citation: Gomez, M., Burow, G.B., Burke, J.J., Burow, M., Denwar, N., Simpson, C., Ramasubramanian, T., Puppala, N. 2008. Identification of peanut hybrids using microsatellite markers and horizontal polyacrylamide gel electrophoresis. Peanut Science. 35(2):123-129.

Interpretive Summary: In peanut a self pollinating crop, hybridization is quite time consuming and challenging. A major problem is the identification of selfs form hybrids after crossing. To aid in distinguish selfs from hybrids, DNA was extracted from leaf tissue of F1 or F2 plants, and SSR markers were amplified and bands separated by submarine horizontal polyacrylamide gel electrophoresis (H-PAGE). By comparison of banding patterns to parents, 82% of the putative hybrids were demonstrated to be true hybrids on the basis of possessing a marker allele from the male parent. The H-PAGE gels gave better band separation and differentiation of selfed progenies than agarose gels, and were compatible with the common horizontal agarose gel units. This method provides a quick assay to distinguish hybrids from inadvertent selfs, resulting in a saving of resources otherwise used on propagation of selfs.

Technical Abstract: In peanut hybridization, it is very important to be able to distinguish selfs from true hybrids to save time and resources. To help facilitate this effort and provide molecular distinction between selfs from hybrids, DNA was extracted from leaf tissue of F1 or F2 plants, and SSR markers were amplified and bands separated by submarine horizontal polyacrylamide gel electrophoresis (H-PAGE). By comparison of banding patterns to parents, 82% of the putative hybrids were demonstrated to be true hybrids on the basis of possessing a marker allele from the male parent. The H-PAGE gels gave better band separation and differentiation of selfed progenies than agarose gels, and were compatible with the common horizontal agarose gel units. This method provides a quick assay to distinguish hybrids from inadvertent selfs, resulting in a saving of resources otherwise used on propagation of selfs.