Title: Identification of genes in Puccinia striiformis and use of gene-specific PCR primers to differentiate rust species, formae speciales, and isolates Authors
|Wang, M - WASHINGTON STATE UNIV|
|Ling, P - FORMER USDA-ARS|
Submitted to: National American Phytopathology Meetings
Publication Type: Abstract Only
Publication Acceptance Date: June 1, 2006
Publication Date: July 1, 2007
Citation: Wang, M.N., Ling, P., Chen, X. 2006. Identification of genes in Puccinia striiformis and use of gene-specific PCR primers to differentiate rust species, formae speciales, and isolates. APS Pacific Division Mtg, June 13-16, Boise, ID. Phytopathology 97:S171. Technical Abstract: Stripe rust, caused by Puccinia striiformis (P. s.), is an important disease on wheat, barley, and grasses. To study functional genomics, we have constructed a full-length cDNA library from urediniospores of P. s. f. sp. tritici. Of 196 sequenced cDNA clones, 37.8% had significant homology to the sequences of genes with characterized functions, 16.8% had significant homology to hypothetical proteins, 19.4% had some homology with genes, and 26.0% did not have significant homology with any sequences in other fungi. A total of 51 different protein products were identified and they are involved in growth, defense, and virulence/infection. In order to develop specific primers to differentiate rust species, formae speciales, and isolates, 28 gene-specific primer pairs were designed based on sequences of some of the identified genes. Using these primers, we have identified polymorphic markers to distinguish P. s. from P. graminis, P. triticina, and P. hordei, and markers to separate P. s. f. sp. tritici from P. s. f. sp. hordei, P. s. f. sp. poae, and P. s. f. sp. dactylidis. We also identified primers to detect polymorphisms among isolates of P. s. f. sp. tritici.