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Title: Genetic characterization and QTL mapping of high-temperature, adult-plant resistance in ‘Bancroft’ barley against Puccinia striiformis f. sp. hordei

Author
item YAN, G - WASHINGTON STATE UNIV
item Chen, Xianming

Submitted to: National American Phytopathology Meetings
Publication Type: Abstract Only
Publication Acceptance Date: 6/1/2006
Publication Date: 7/1/2006
Citation: Yan, G.P., Chen, X. 2006. Genetic characterization and QTL mapping of high-temperature, adult-plant resistance in ‘Bancroft’ barley against Puccinia striiformis f. sp. hordei. APS Pacific Division Meeting, June 13-16, Boise, ID. Phytopathology 97:S171

Interpretive Summary:

Technical Abstract: Bancroft barley has been identified to have durable high-temperature adult-plant resistance (HTAP) to stripe rust (Puccinia striiformis f. sp. hordei). To map quantitative trait loci (QTL) for the HTAP resistance, Bancroft was crossed with susceptible Harrington barley. The parents and F4 and F5 progeny of the crosses were evaluated in three locations in 2004 and 2005, respectively. Infection type (IT) and disease severity (DS) were recorded three times during the growing season. Area under disease progress curve (AUDPC) was calculated for each line based on the DS data. Analysis of variance with the IT data showed no significant differences among the locations, indicating that the HTAP resistance is non-race specific. A linkage map was constructed with eight resistance gene analog polymorphism (RGAP) markers and mapped on the long arm of barley chromosome 3H with three microsatellite markers identified using 119 F6 lines. QTL mapping identified a single gene that explained 95%, 76%, and 71% of the total variations using the IT, DS, and AUDPC data, respectively. The two flanking RGAP markers detected polymorphism in 23 of 26 barley cultivars, indicating that the markers are useful to incorporate the HTAP resistance into most of the cultivars.