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ARS Home » Southeast Area » Stoneville, Mississippi » Warmwater Aquaculture Research Unit » Research » Publications at this Location » Publication #223578
Title: TLR5, NRAMP, TNF, AND Hepcidin Response to Challenge with Edwardsiella ictaluri in Channel Catfish Families with High and Low Susceptibility to Infection

Author
item Booth, Natha
item Peterson, Brian

Submitted to: Aquaculture
Publication Type: Abstract Only
Publication Acceptance Date: 1/15/2008
Publication Date: 2/11/2008
Citation: Booth, N.J., Peterson, B.C. 2008. TLR5, NRAMP, TNF, AND Hepcidin Response to Challenge with Edwardsiella ictaluri in Channel Catfish Families with High and Low Susceptibility to Infection. Aquaculture America 2008, Abstracts, p. 494, Orlando, FL. USA.

Interpretive Summary:

Technical Abstract: Responses of toll-like receptor 5 (TLR5), tumor necrosis factor (TNF), natural resistance-associated macrophage protein (Nramp), and hepcidin to experimental challenge with Edwardsiella ictaluri in two families of channel catfish were measured in order to understand the mechanisms through which E. ictaluri causes infection. One family demonstrated high susceptibility to infection with E. ictaluri in previous challenges (>90% mortality), while the other family demonstrated very low susceptibility (<20% mortality). Fish were challenged by immersion with virulent E. ictaluri and sampled at 6, 24, 48, and 72 hrs post-challenge. Levels of mRNA expression of TLR5, TNF, Nramp, and hepcidin in liver tissue were measured by quantitative RT-PCR. TNF and TLR5 expression in liver increased significantly at 48 hrs post-challenge in both the high and low susceptibility families (p