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United States Department of Agriculture

Agricultural Research Service

Research Project: DEVELOPMENT AND APPLICATION OF MOLECULAR PHYLOGENETICS OF FUNGI TO ENHANCE FOOD SAFETY AND FOOD SECURITY

Location: Bacterial Foodborne Pathogens & Mycology Research Unit

Title: A two-locus DNA sequence database for identifying host-specific pathogens and phylogenetic diversity within the Fusarium oxysporum species complex

Authors
item O`donnell, Kerry
item Johnston, P - LANDCARE RES, NW ZEALAND
item Crous, P - CBS, UTRECTH, NETHERLANDS
item Gueidan, C - CBS, UTRECTH, METHERLANDS
item Colyer, P - LA STATE UNIV AG CNTR
item Glenn, Anthony
item Riley, Ronald
item Zitomer, Nicholas
item Waalwijk, C - WAGENINGEN UNIV & RES
item Geiser, D - PENN STATE UNIV PA
item Kang, S - PENN STATE UNIV PA
item Juba, J - PENN STATE UNIV PA
item Sutton, D - UNIV OF TX HLTH SCI CNTR
item Ploetz, P - UNIV OF FL HOMESTEAD FL
item Elliot, M - UNIV OF FL FT LAUDERDALE
item Kistler, H
item Davis, M - UNIV OF CA DAVIS
item Sink, Stacy
item Sarver, B - WA UNIV IN ST LOUIS

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: September 2, 2008
Publication Date: September 2, 2008
Citation: O Donnell, K., Johnston, P., Crous, P., Gueidan, C., Colyer, P., Glenn, A.E., Riley, R.T., Zitomer, N.C., Waalwijk, C., Geiser, D., Kang, S., Juba, J., Sutton, D.A., Ploetz, P., Elliot, M., Kistler, H.C., Davis, M., Sink, S.L., Sarver, B. 2008. A two-locus DNA sequence database for identifying host-specific pathogens and phylogenetic diversity within the Fusarium oxysporum species complex. Meeting Abstract.

Technical Abstract: An electronically portable two-locus DNA sequence database, comprising partial sequences of the translation elongation factor gene (EF-1a, 634 bp alignment) and nearly complete sequences of the nuclear ribosomal intergenic spacer region (IGS rDNA, 2220 bp alignment) for 850 isolates spanning the phylogenetic breadth of the Fusarium oxysporum species complex (FOSC), was constructed to investigate evolutionary relationships and provide discrete nucleotide sequence-based markers for identifying host-specific pathogens (i.e., formae speciales), putative non-pathogens, and opportunistic pathogens of humans and other animals within the FOSC. Of the 850 isolates subjected to the DNA-sequence based typing scheme, 101 EF-1a, 204 IGS rDNA, and 257 two-locus sequence types (STs) were differentiated. One hundred and forty of the two locus STs were singletons. The human mycoses-associated isolates were widely distributed throughout the FOSC phylogeny and represent at least 25 putatively clonal lineages. Several of these lineages are exclusively associated with mycotic infections, whereas others include isolates from hospital or industrial plumbing systems. Mycotoxin analyses of twenty isolates representing the phylogenetic breadth of the FOSC indicate that moniliformin (MON) and fumonisin (FUM) production may be very limited in that only one strain produced MON and one FUM. This data will be incorporated into the next version of the web-accessible Fusarium-ID database.

Last Modified: 9/21/2014
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