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United States Department of Agriculture

Agricultural Research Service

Research Project: MINIMIZING THE ADVERSE HEALTH AND ECONOMIC IMPACTS OF MYCOTOXINS AND PLANT TOXINS IN FOODS Title: A single extraction method for the analysis by liquid chromatography/tandem mass spectrometry of fumonisins and biomarkers of disrupted sphingolipid metabolism in tissues of maize seedlings

Authors
item Zitomer, Nicholas
item Glenn, Anthony
item Bacon, Charles
item Riley, Ronald

Submitted to: Mycological Society of America
Publication Type: Abstract Only
Publication Acceptance Date: June 16, 2008
Publication Date: August 9, 2008
Citation: Zitomer, N.C., Glenn, A.E., Bacon, C.W., Riley, R.T. 2008. A single extraction method for the analysis by liquid chromatography/tandem mass spectrometry of fumonisins and biomarkers of disrupted sphingolipid metabolism in tissues of maize seedlings. Mycological Society of America. August 9 - 13, 2008. State College, PA.

Interpretive Summary: Abstract - no summary required

Technical Abstract: The fungus Fusarium verticillioides is a pathogen of many plants and is known to produce fumonisins. These toxins have been shown to contribute to the development of maize seedling disease. Fumonisin disruption of sphingolipid biosynthesis has been demonstrated to occur during such pathogenesis. A liquid chromatographic/mass spectrometric method was developed for the analysis of fumonisin content in maize leaf tissue, as well as the elevation of biomarkers of sphingoid base disruption in those tissues. This method involved a quick extraction and subsequent analysis on a mass spectrometer. To test the efficacy of the method, seed of susceptible and resistant maize lines were inoculated with a pathogenic, fumonisin-producing strain of F. verticillioides. The maize seedlings were then harvested, and analyzed for fumonisin, as well as sphingoid bases and their 1-phosphates. Fumonisin accumulation was evident in the leaves of inoculated plants and was significantly greater in the leaves of the susceptible maize variety than the resistant variety, as was elevation of sphingoid bases and sphingoid base 1-phosphates. Unexpectedly, FB1 was preferentially accumulated in the leaf tissues over FB2 and FB3. The method developed was effective, fast, and sensitive for use in determining these indicators of disease induced by infection and toxin production.

Last Modified: 11/22/2014
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