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United States Department of Agriculture

Agricultural Research Service

Research Project: APPLICATION OF BIOLOGICAL AND MOLECULAR TECHNIQUES TO THE DIAGNOSIS AND CONTROL OF AVIAN INFLUENZA AND OTHER EMERGING POULTRY PATHOGENS Title: Use of genomic interspecies microarray hybridization to detect differentially expressed genes associated with H5N1 avian influenza virus infections in ducks

Authors
item Pantin-Jackwood, Mary
item Sarmento, Luciana
item Afonso, Claudio

Submitted to: American Association of Veterinary Laboratory Diagnosticians
Publication Type: Abstract Only
Publication Acceptance Date: August 21, 2008
Publication Date: October 23, 2008
Citation: Pantin Jackwood, M.J., Sarmento, L., Afonso, C.L. 2008. Use of genomic interspecies microarray hybridization to detect differentially expressed genes associated with H5N1 avian influenza virus infections in ducks [abstract]. Proceedings of the American Association of Veterinary Laboratory Diagnosticians Annual Meeting, October 23-29, 2008, Greensboro, NC. 2008 CDROM.

Technical Abstract: The Asian H5N1 highly pathogenic avian influenza (HPAI) viruses have changed from producing mild respiratory infections in ducks, to some strains producing severe disease and mortality. The objective of this study was to examine the differences in host response to infection with H5N1 HPAI viruses with different pathogenicity in ducks by determining gene expression in tissues of infected ducks using a chicken genome microarray. The use of cDNA microarrays offers a highly effective system to study transcriptional responses during host-pathogen interaction. By using genomic interspecies microarray hybridization we can detect a large number of genes, provided that the microarray for a fully sequenced close relative is available. A 44K, 60-mer oligonucleotide, whole chicken genome microarray was used to compare gene expression in spleens from Pekin ducks infected with two different HPAI viruses, A/Ck/HK/220/97 and A/Egret/HK/757.2/02. An important number of differentially expressed genes associated with infection were detected and demonstrated the complexity of the patterns of gene expression in ducks in response to HPAI. Semi-quantitative RT-PCR was used to confirm the regulated expression of several of the differentially expressed genes. The results obtained suggest that different mechanisms are potentially induced by avian influenza viruses to modulate the host response to infection. The differentially expressed genes identified in this study are candidates for further hypothesis-driven investigation of the mechanisms involved in resistance to AI viruses in ducks.

Last Modified: 11/24/2014
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