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United States Department of Agriculture

Agricultural Research Service

Research Project: SUSTAINABLE VINEYARD PRODUCTION SYSTEMS

Location: Crops Pathology and Genetics Research

Title: Microsatellite Markers for the Grapevine Pathogen, Eutypa Lata.

Authors
item Baumgartner, Kendra
item Bergemann, Sarah - MIDDLE TENNESSEE ST. UNIV
item Fujiyoshi, Phillip
item Rolshausen, Philippe - UNIV CONNECTICUT
item Gubler, W. - UNIV CALIFORNIA, DAVIS

Submitted to: Molecular Ecology Resources
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: June 10, 2008
Publication Date: October 21, 2008
Repository URL: http://www3.interscience.wiley.com/cgi-bin/fulltext/121476700/PDFSTART
Citation: Baumgartner, K., Bergemann, S.E., Fujiyoshi, P.T., Rolshausen, P.E., Gubler, W.D. 2008. MICROSATELLITE MARKERS FOR THE GRAPEVINE PATHOGEN, EUTYPA LATA.. Molecular Ecology Resources. 9:222-224.

Interpretive Summary: The means of spread of Eutypa dieback from vine-to-vine within vineyards is likely due to dispersal of Eutypa lata sexual spores (ascospores), and not asexual spores (conidia), based on evidence of distributions of vegetative compatibility groups, reproductive structures (perithecia), and symptomatic grapevines. Although it seems clear that ascospores initiate infections of vines, the origin of ascospores that initiate the first infections in a healthy vineyard is not clear. Possible sources include distant vineyards, forest trees, or apricot orchards. To evaluate the relatedness of E. lata populations from vineyards, forests, and apricot orchards, we isolated and characterized nine E. lata-specific microsatellite markers, in populations from two California vineyards (24 isolates per vineyard). Allele frequency ranged from two to 11 alleles per locus and haploid gene diversity ranged from 0.33 to 0.83. All samples comprised unique haplotypes. Our results suggest that there is sufficient allelic polymorphism to estimate fine-scale spatial structure, and to identify parent-sibling pairs and possible sources of inoculum from outside sources.

Technical Abstract: We isolated and characterized nine polymorphic microsatellite markers for Eutypa lata, a fungal pathogen responsible for Eutypa dieback of grapevine, in populations from two California vineyards (24 isolates per vineyard). Allele frequency ranged from two to 11 alleles per locus and haploid gene diversity ranged from 0.33 to 0.83. All samples comprised unique haplotypes. Our results suggest that there is sufficient allelic polymorphism to estimate fine-scale spatial structure, and to identify parent-sibling pairs and possible sources of inoculum from outside sources.

Last Modified: 7/30/2014
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