Page Banner

United States Department of Agriculture

Agricultural Research Service

Title: Revealing of Biological Activity in Crude Extracts, Seperated Fractions, Groups of Chemical Substance and Individual Compounds

Authors
item Cantrell, Charles
item Mamonov, L.K. - INST. PLT BIO & BIOTECH
item Vassilyev, J.I. - INST. PLT BIO & BIOTECH
item Mukanova, G.S. - INST. PLT BIO & BIOTECH
item Achmetova, D.S. - INST. PLT BIO & BIOTECH

Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: April 15, 2008
Publication Date: April 15, 2008
Citation: Cantrell, C.L., Mamonov, L., Vassilyev, J., Mukanova, G., Achmetova, D. 2008. Revealing of Biological Activity in Crude Extracts, Seperated Fractions, Groups of Chemical Substance and Individual Compounds. Book Chapter. In: Introduction to Phytochemical Investigations and Discovering Biological Activities of Phytogenous Substances. Eds. B.M. Butin and B.A. Sarsenbaev. Institute of Plant Biology and Biotechnology, Almaty, Kazakhstan. pp. 127-157.

Interpretive Summary: Crude extracts, separated fractions, groups of chemical substances, and individual compounds from natural sources are all evaluated stepwise while performing purifications in in-house bioassays. In a stepwise fashion proceeding from crude extracts to fractions and on to pure compounds, decisions are made regarding how best to proceed based on biological evaluation results at each step. In order to use this bioassay-guided approach, the bioassays bust not only be relatively quick but such bioassays must also be capable of quantitatively ranking activity on sub milligram quantities of material. Natural product purifications often require the ability to work with such small sample sizes for both chemical and biological evaluations.

Technical Abstract: Crude extracts, separated fractions, groups of chemical substances, and individual compounds from natural sources are all evaluated stepwise while performing purifications in in-house bioassays. In a stepwise fashion proceeding from crude extracts to fractions and on to pure compounds, decisions are made regarding how best to proceed based on biological evaluation results at each step. In order to use this bioassay-guided approach, the bioassays bust not only be relatively quick but such bioassays must also be capable of quantitatively ranking activity on sub milligram quantities of material. Natural product purifications often require the ability to work with such small sample sizes for both chemical and biological evaluations.

Last Modified: 10/1/2014
Footer Content Back to Top of Page