Location: Aquatic Animal Health Research
Title: Molecular Cloning and Sequencing of Channel Catfish, Ictalurus punctatus, Cathepsin H and L cDNA Authors
Submitted to: Genbank
Publication Type: Other
Publication Acceptance Date: July 30, 2008
Publication Date: August 23, 2008
Citation: Yeh, H., Klesius, P.H. 2008. Molecular Cloning and Sequencing of Channel Catfish, Ictalurus punctatus, Cathepsin H and L cDNA. Genbank. Accession Nos. EU915299, EU915298. Technical Abstract: Cathepsin H and L, a lysosomal cysteine endopeptidase of the papain family, are ubiquitously expressed and involve in antigen processing. In this communication, the channel catfish cathepsin H and L transcripts were sequenced and analyzed. Total RNA from tissues was extracted and cDNA libraries were constructed by the rapid amplification cDNA end (RACE) method. The gene-specific primers in conjunction with the RACE primers were used to PCR amplify 5’- and 3’-ends of the cathepsin H and L transcripts. The complete channel catfish cathepsin H cDNA comprised 1415 bp including a 978-bp open reading frame, which potentially encodes a putative protein of 326 amino acid residues with calculated molecular mass of 36.6 kDa and pI of 5.51. The complete channel catfish cathepsin L cDNA comprised 1639 nucleotides including a 1008-bp open reading frame, which potentially encodes a putative protein of 336 amino acid residues with calculated molecular mass of 38.1 kDa and pI of 5.84.