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United States Department of Agriculture

Agricultural Research Service

Research Project: MOLECULAR ANALYSIS OF VIRULENCE DETERMINANTS OF SELECT BACTERIA IN FISH DISEASES

Location: Aquatic Animal Health Research

Title: Molecular Cloning and Sequencing of Channel Catfish, Ictalurus punctatus, Cathepsin H and L cDNA

Authors
item Yeh, Hung-Yueh
item Klesius, Phillip

Submitted to: Genbank
Publication Type: Other
Publication Acceptance Date: July 30, 2008
Publication Date: August 23, 2008
Citation: Yeh, H., Klesius, P.H. 2008. Molecular Cloning and Sequencing of Channel Catfish, Ictalurus punctatus, Cathepsin H and L cDNA. Genbank. Accession Nos. EU915299, EU915298.

Technical Abstract: Cathepsin H and L, a lysosomal cysteine endopeptidase of the papain family, are ubiquitously expressed and involve in antigen processing. In this communication, the channel catfish cathepsin H and L transcripts were sequenced and analyzed. Total RNA from tissues was extracted and cDNA libraries were constructed by the rapid amplification cDNA end (RACE) method. The gene-specific primers in conjunction with the RACE primers were used to PCR amplify 5’- and 3’-ends of the cathepsin H and L transcripts. The complete channel catfish cathepsin H cDNA comprised 1415 bp including a 978-bp open reading frame, which potentially encodes a putative protein of 326 amino acid residues with calculated molecular mass of 36.6 kDa and pI of 5.51. The complete channel catfish cathepsin L cDNA comprised 1639 nucleotides including a 1008-bp open reading frame, which potentially encodes a putative protein of 336 amino acid residues with calculated molecular mass of 38.1 kDa and pI of 5.84.

Last Modified: 9/10/2014
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