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United States Department of Agriculture

Agricultural Research Service

Research Project: GENOME-BASED STRATEGIES FOR DETECTION AND IDENTIFICATION OF PLANT PATHOGENIC PHYTOPLASMAS AND SPIROPLASMAS Title: Identification of Phytoplasma Species Associated With Potato Diseases in Russia

Authors
item Girsova, N - MOSCOW RUSSIA
item BOTTNER, KRISTI
item Mozhaeva, K - MOSCOW RUSSIA
item Kastalyeva, T - MOSCOW RUSSIA
item Owens, Robert
item LEE, ING MING

Submitted to: International Symposium on Crop Protection
Publication Type: Proceedings
Publication Acceptance Date: August 15, 2008
Publication Date: August 30, 2008
Citation: Girsova, N., Bottner, K.D., Mozhaeva, K.A., Kastalyeva, T.B., Owens, R.A., Lee, I. 2008. Identification of Phytoplasma Species Associated With Potato Diseases in Russia. International Symposium on Crop Protection. vol. 73(2). pp. 331-333.

Technical Abstract: Four out of six known potato diseases attributed to phytoplasma infection were previously reported to occur in Russia based on a combination of biological properties such as symptomatology and/or vector relationships and electron microscopy of infected phloem tissue. In 2007, the first molecular identification of potato diseases causing symptoms including purple top, round leaves, stunting, bud proliferation and formation of aerial tubers was carried out using PCR methods. A nested PCR using primer pair P1/P7 in the first amplification followed by R16F2n/R16R2n in the second amplification was performed to detect phytoplasmas in infected potato samples. PCR products were digested singly with several restriction enzymes. Comparison of RFLP profiles with published profiles was used for identification of the putative phytoplasmas detected. The majority of 49 PCR positive potato samples showed RFLP profiles of 16S rDNA sequences very similar or identical to stolbur phytoplasma, a strain belonging to stolbur phytoplasma group (16Sr XII), subgroup 16SrXII-A, and only two showed RFLP profiles similar to those of aster yellow phytoplasma strains (‘Candidatus Phytoplasma asteris’) belonging to aster yellows phytoplasma group (16SrI), subgroup 16SrI-A and 16SrI-B. The results demonstrated that stolbur phytoplasma is prevalent in several potato growing regions of Russia.

Last Modified: 7/28/2014
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