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United States Department of Agriculture

Agricultural Research Service

Research Project: GENETIC ENHANCEMENT FOR RESISTANCE TO BIOTIC AND ABIOTIC STRESSES IN HARD WINTER WHEAT

Location: Hard Winter Wheat Genetics Research Unit

Title: A BAC-based physical map of the Hessian fly (Mayetiola destructor) genome anchored to polytene chromosomes

Authors
item Aggarwal, Rajat - PURDUE UNIVERSITY
item Benatti, Thiago - PURDUE UNIVERSITY
item Gill, Navdeep - PURDUE UNIVERSITY
item Chen, Ming-Shun
item Schemerhorn, Brandon
item Fellers, John
item Stuart, Jeff - PURDUE UNIVERSITY

Submitted to: Biomed Central (BMC) Genomics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: June 23, 2009
Publication Date: July 5, 2009
Citation: Aggarwal, R., Benatti, T., Gill, N., Chen, M., Schemerhorn, B.J., Fellers, J.P., Stuart, J.J. 2009. A BAC-based physical map of the Hessian fly (Mayetiola destructor) genome anchored to polytene chromosomes. Biomed Central (BMC) Genomics. 10:293.

Interpretive Summary: The Hessian fly (Mayetiola destructor) is one of the most destructive insects of wheat world-wide. Resistant wheat is the best strategy to control the damage caused by this pest. The challenge for the use of resistant wheat is the ability of the insect to develop new populations that can overcome resistance once a resistant wheat is deployed to the field. To find how the insect can overcome wheat resistance, the genes that are responsible for the Hessian fly to attack wheat need to be identified. This work is to provide a detailed map of the Hessian fly genome with specific markers. The work provides a foundation for identification of specific Hessian fly genes and for whole genome sequencing.

Technical Abstract: The Hessian fly (Mayetiola destructor) is an important insect pest of wheat and an experimental organism for studies of plant-insect interactions. It has tractable genetics, polytene chromosomes, a relatively small genome (158 Mb), and shares a gene-for-gene relationship with wheat. To improve its capacity as a model plant-gall forming insect, an FPC-based genome wide physical map of the Hessian fly was constructed and anchored to the polytene chromosomes of the insect. Bacterial artificial chromosome (BAC) clones corresponding to 10.5-fold coverage of the Hessian fly genome were fingerprinted, using high-resolution capillary polyacrylamide gel electrophoresis, and end sequenced. The reliability of the FPC assembly was tested using fluorescence in situ hybridization (FISH) to co-localize two BAC clones from each of the 196 longest contigs on the polytene chromosomes. To assess contig distribution, 70 additional contigs were FISH mapped. The FISH mapped contigs were evenly distributed and covered 60% of the genome (95,668 kb). Only 3.63% of the BAC-end sequence was composed of transposable elements, helicases, ribosomal repeats, simple sequence repeats, and sequences of low complexity. However, 14.51% of the BES was comprised of multi-copy gene sequences. This physical map provides the foundation for high-resolution genetic mapping, map-based cloning, and assembly of complete genome sequencing data. The Hessian fly BAC clone assembly, and the names and positions of the BAC clones used in the FISH experiments are freely available at http://genome.purdue.edu/WebAGCoL/Hfly/WebFPC/.

Last Modified: 9/1/2014
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