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United States Department of Agriculture

Agricultural Research Service

Research Project: Germplasm Enhancement and Genetic Improvement of Cotton

Location: Genetics and Precision Agriculture Research

Title: Chromosomal assignment of ALFP markers in upland cotton (Gossypium hirsutum L.)

Authors
item Myers, Geroald - LOUISIANA STATE UNIV
item Jiang, Baogong - LOUISIANA STATE UNIV
item Akash, Muhanad - UNIVERSITY OF JORDAN
item Badigannavar, Ashok - LOUISIANA STATE UNIV
item Saha, Sukumar

Submitted to: Euphytica
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: August 20, 2008
Publication Date: April 1, 2009
Citation: Myers, G.M., Jiang, B., Akash, M.W., Badigannavar, A., Saha, S. 2009. Chromosomal assignment of ALFP markers in upland cotton (Gossypium hirsutum L.). Euphytica. 165:391-399.

Interpretive Summary: DNA markers are valuable tools for the detection, mapping and estimation of gene effects of important agronomic traits. Amplified fragment length polymorphism (AFLP) is a DNA fingerprinting technique capable of detecting several markers at one time. Identification of informative AFLP markers specific to different chromosomes will help in understanding the structure, organization, evolution and function of the cotton genome. In this research, we used two sets of cotton aneuploid (G. hirsutum × G. tomentosum and G. hirsutum × G. barbadense) plants, deficient for specific chromosome or chromosome arm, to locate AFLP markers to chromosome or chromosome arm using deletion analysis method. Thirty-eight primer combinations were used to generate 608 polymorphic AFLP markers. Ninety-eight AFLP markers were assigned to 22 different cotton chromosomes or chromosome arms. Based on the 16 common AFLP markers, we were able to associate 13 linkage groups, developed previously in our lab, to 8 different chromosomes. This research will provide new resources in cotton genomic research and will complement molecular mapping efforts in other labs.

Technical Abstract: In this research, we used two sets of cotton aneuploid (G. hirsutum × G. tomentosum and G. hirsutum × G. barbadense) plants to locate AFLP markers to chromosome using deletion analysis method. Thirty-eight primer combinations were used to generate 608 polymorphic AFLP markers. Ninety-eight AFLP markers were assigned to 22 different cotton chromosomes or chromosome arms. Of those assigned markers, 63.3% were assigned to A genome and 36.7% were assigned to D genome. A low rate (14.3%) of common markers were found between those assigned AFLP markers with the AFLP markers from an intraspecific cross populations developed previous in our lab. Based on the 16 common markers, we were able to associate 13 linkage group developed previously in our lab to 8 chromosomes. Further research will be carried out by using SSR markers with known location to associated unassigned linkage group to chromosome

Last Modified: 9/10/2014
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