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United States Department of Agriculture

Agricultural Research Service

Title: Controllable Biotinylated Poly(Ethylene-co-Glycidyl Methacrylate) (PE-co-GMA) Nanofibers to Bind Streptavidin-Horseradish Peroxidase (HRP) for Potential Biosensor Applications

Authors
item Wang, Dong - UC DAVIS
item Sun, Gang - UC DAVIS
item Xiang, Bei - UC DAVIS
item Chiou, Bor-Sen

Submitted to: European Polymer Journal
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: April 18, 2008
Publication Date: July 1, 2008
Citation: Wang, D., Sun, G., Xiang, B., Chiou, B. 2008. Controllable Biotinylated Poly(Ethylene-co-Glycidyl Methacrylate) (PE-co-GMA) Nanofibers to Bind Streptavidin-Horseradish Peroxidase (HRP) for Potential Biosensor Applications. European Polymer Journal. 2008. 44(7):2032-2039.

Interpretive Summary: A novel extrusion process was used to make polymer nanofibers that can potentially be used for biosensors. The surface of these nanofibers were chemically modified to immobilize bioactive enzymes. The enzymes showed high reactivity and efficiency. Also, the nanofibers still retained relatively high activity after multiple uses, indicating good potential for reusability.

Technical Abstract: Poly(ethylene-co-glycidyl methacrylate) (PE-co-GMA) nanofibers with abundant active epoxy groups on surfaces were fabricated through a novel manufacturing process. The prepared PE-co-GMA nanofibers with different average diameters ranging from 100 to 400 nm were aminated by reacting the epoxy groups with 1,3-diaminopropane. The resulting aminated PE-co-GMA nanofibers were subsequently biotinylated and then successfully applied to immobilize streptavidin–horseradish peroxidase (HRP) conjugate via specific, strong and rapid binding of biotin and streptavidin. The streptavidin–HRP immobilized PE-co-GMA nanofibers showed high activity, efficiency, sensitivity as well as good reusability. The results demonstrated that PE-co-GMA nanofibers prepared could be a promising candidate as solid support materials for potential biosensor applications.

Last Modified: 12/19/2014
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