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United States Department of Agriculture

Agricultural Research Service

Research Project: REDUCING THE ALLERGENIC PROPERTIES OF PEANUTS Title: Epitope analysis of peanut allergen Ara h1 with oligoclonal IgM antibody from human B-lymphoblastoid cells.

Authors
item Naganawa, Yasunori - INST. BIOSCIENCE & TECH
item Shimmoto, Michie - TOKYO INST. MEDICAL SCI
item Maleki, Soheila
item Takase, Masayuki - NATL. FOOD RESEARCH
item Shinmoto, Hiroshi - NATL. FOOD RESEARCH

Submitted to: Cytotechnology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: March 11, 2008
Publication Date: June 1, 2008
Citation: Naganawa, Y., Shimmoto, M., Maleki, S.J., Takase, M., Shinmoto, H. 2008. Epitope analysis of peanut allergen Ara h1 with oligoclonal IgM antibody from human B-lymphoblastoid cells. Cytotechnology. 9142-9143.

Interpretive Summary: To identify the location of the binding sites on a major peanut allergy by human antibodies, human immune cells were cultured. The antibodies they produced were harvested and analyzed for binding to Ara h 1, a major peanut allergen. A unique antibody binding area was identified between amino acid 171 and 230 of the allergen and was named “area c”.

Technical Abstract: To analyze epitopes of peanut allergen Ara h1, Epstein-Barr virus-transformed human peripheral oligoclonal B-cells were cultured to obtain antibodies to Ara h1. The combined reaction pattern with six oligoclonal antibodies showed there were six antibody binding areas named a to f in Ara h1. We found the novel antibody binding area named ‘‘area c’’ (171–230aa).

Last Modified: 10/30/2014
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