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United States Department of Agriculture

Agricultural Research Service

Research Project: DEVELOPMENT AND EVALUATION OF IMPROVED MATERIALS FOR MYCOTOXIN ANALYSIS Title: Effect of Cyclodextrins on Spectroscopic Properties of Fluorescent Derivatives of T-2 Toxin

Authors
item Verrone, R - UNIVERSITY OF BARI
item Catucci, L - UNIV OF BARI/IPCF-CNR
item Agostiano, A - UNIV OF BARI/IPCF-CNR
item Lippolis, V - INS OF SCI OF FOOD PROD
item Pascale, M - INS OF SCI OF FOOD PROD
item Maragos, Chris

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: September 11, 2008
Publication Date: September 7, 2008
Citation: Verrone, R., Catucci, L., Agostiano, A., Lippolis, V., Pascale, M., Maragos, C.M. 2008. Effect of Cyclodextrins on Spectroscopic Properties of Fluorescent Derivatives of T-2 Toxin [abstract]. XIII International Symposium on Luminescence Spectrometry. PO151.

Technical Abstract: T-2 toxin is a Fusarium mycotoxin that can occur in several cereals and cereal-based products. It is a potent inhibitor of DNA, RNA and protein synthesis, and shows immunosuppressive and cytotoxic effects both in vivo and in vitro. EU maximum admissible levels for T-2 (and HT-2) toxin in unprocessed cereals and cereal products are currently under discussion. There is a need to develop sensitive and accurate analytical methods for determining this mycotoxin in order to properly assess the relevant risk of human exposure. 1-anthroylnitrile (1-AN) and pyrene-1-carbonyl cyanide (PCC) have been recently shown to be efficient labeling reagents for the determination of T-2 toxin by high-performance liquid chromatography (HPLC) with fluorescence detection. Interactions between mycotoxins and CDs have been shown to produce fluorescence enhancement which could be exploited to improve mycotoxin detection. In this work, the effect of different CDs on spectroscopic properties of T-2 fluorescent derivatives with 1-AN and PCC in several solvents has been investigated by means of steady-state fluorescence and UV-Vis absorption. Data showed that heptakis-2,6-di-O-methyl-ß-CD (DIMEB) caused the highest enhancement in fluorescence emission of labeled T-2 toxin in pure water solutions. Binding constants and complex stoichiometry of T-2 derivatives with ß-CDs were determined by fluorescence measurement applying the modified Benesi-Hildebrand equation. In addition, thermodynamic parameters were obtained from vant’Hoff plots. The application of DIMEB for detecting T-2 toxin in cereals by HPLC with fluorescence detection has been evaluated.

Last Modified: 10/23/2014
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