Page Banner

United States Department of Agriculture

Agricultural Research Service

Research Project: APPLICATION OF BIOLOGICAL AND MOLECULAR TECHNIQUES TO THE DIAGNOSIS AND CONTROL OF AVIAN INFLUENZA AND OTHER EMERGING POULTRY PATHOGENS Title: Effects of specific amino acid changes on the antigenicity of hemagglutinin molecules of avian influenza isolates from Mexico

Authors
item Pfeiffer, Jennifer
item Lee, Chang Won - OHIO STATE UNIVERSITY
item Jadhao, Samadhan - USDA, FAS, OIRP
item Suarez, David

Submitted to: American Association of Avian Pathologists
Publication Type: Abstract Only
Publication Acceptance Date: January 15, 2009
Publication Date: July 11, 2009
Citation: Pfeiffer, J., Lee, C., Jadhao, S., Suarez, D.L. 2009. Effects of specific amino acid changes on the antigenicity of hemagglutinin molecules of avian influenza isolates from Mexico [abstract]. American Association of Avian Pathologists Annual Meeting, July 11-15, 2009, Seattle, Washington. CD-ROM, Paper No. 7584.

Technical Abstract: Amino acid (aa) changes between the hemagglutinin (HA) proteins of a vaccine avian influenza virus and more recent field isolates were detected following prolonged vaccination of Mexican poultry. Using site-directed mutagenesis and reverse genetics (rg), viruses containing identical backbones but different HAs were generated. The cross-hemagglutination inhibition (HI) and virus neutralization tests were used to compare antigenic differences. A vaccine-challenge study on chickens was performed to compare differences in viral shedding between groups, as determined through real-time reverse transcriptase polymerase chain reaction (RT-PCR) on oropharyngeal swab sample ribonucleic acid (RNA). The goal was to gain knowledge for selecting vaccine seed strains.

Last Modified: 11/23/2014
Footer Content Back to Top of Page